60+ TOP Biochemistry Interview Questions and Answers [UPDATED]

If you’re preparing for Biochemistry job interview and whether you’re experienced or fresher & don’t know what kind of questions will be asked in Biochemistry interview, then go through the below Real Time 60+ Top Biochemistry Interview Questions and Answers to crack your job interview.

Biochemistry Interview Questions and Answers

  • Question: Give The Example For Electrophilic Substitution Reaction.

    Answer :

    The species, which accepts the electrons, are called Electrophilles (or) Electrophilic reagents.

    When the atom (or) group of atoms present in the organic compound is replaced by another atom (or) group of atoms (electrophilic) is called electrophilic substitution reaction.

  • Question: Alkaline Metals When Dissolved In Ammonium (nh3) Act As Better Conductor And Better Reducing Agent What Is The Reason Behind It?

    Answer :

    Due to formation of solvated or Ammoniated electrons

  • Question: Covalent Bond Is Directional Therefore Which Covalent Property Is Shown?

    Answer :

    Space Isomerism

  • Question: Define Bond Angle.

    Answer :

    The angle between the lines joining the nuclei of the bonded atom with a central atom is called bond angle.

  • Question: Define Bond Energy.

    Answer :

    The amount of energy released when one mole of bonds are formed between the corresponding gaseous atoms is called Bond energy.

  • Question: Define Co-¬ordination Covalent Bond.

    Answer :

    Co¬ordination covalent bond is formed by the mutual sharing of pair of electrons between two atoms contributed by only one of the combining atoms.

  • Question: Define Hybridization.

    Answer :

    The distribution of electrons into Hybrid orbitals is as per the Paulis Exclusion principle and the Hunds rule of Maximum Multiplicity.

  • Question: Define Lattice Energy.

    Answer :

    The energy released when one mole of ionic crystal is formed by the combination of the corresponding gaseous (+ve) and (¬ve) ions brought from infinite distance is called lattice energy.

  • Question: Define Limiting Radius.

    Answer :

    The ratio between the radius of cation and the radius of anion is called Limiting radius.

  • Question: Define Polar Covalent Bond

    Answer :

    The covalent bond formed by the unequal sharing of electrons between the two atoms is called polar covalent bond.

  • Question: Define The Phenomenon Resonance.

    Answer :

    When a molecule is represented by two or more nearly equal structures, which differ in the arrangement of electrons, then the molecule is said to exhibit resonance.

  • Question: Give The Chemical Formula For Borax.

    Answer :

    (Na2) B4 (O7) .10(H2) O

  • Question: Give The Formula For Peral Ash.

    Answer :

    The formula for Peral Ash is K2CO3.

  • Question: How Can Energy Change In The Formation Of Nacl Be Determined?

    Answer :

    With the help of Born Haber’s cycle.

  • Question: How Do We Prepare Diborane?

    Answer :

    The Diborane (B2H6) is prepared by the reduction of BCl3 with aluminium hydride B2H6+3AlCl3+3LiClà4 BCl3+3LiAlH4

  • Question: How Do You Define Free Radical Addition Reaction?give An Example.

    Answer :

    When unsaturated compounds undergo addition reactions with free radicals,it is called free radical addition reaction.

    Ex; CH3¬CH = CH2+HBr———–> CH3¬CH2¬CH2Br

  • Question: How Many Elements Are There In 3d¬ Series Of First Transition Series?

    Answer :

    Ten elements from Sc (Z=21) to Zn (z=30) or {Sc –scandium to Zn (zinc)}.

  • Question: How Many Sigma And Pi Bonds Are Present In Nitrogen Formula?

    Answer :

    Each nitrogen molecule contain one sigma and 2 Pi bonds

  • Question: How Much Amount Of Energy Is Required To Break The Triple Bond In Nitrogen Molecule?

    Answer :

    225 Kcal/mole or 945.4 KJ/mole

  • Question: In Double Bond, How Many Sigma And Pi Bonds Are Present In It?

    Answer :

    Double bond = 1 Sigma bond and one Pi bond Among sigma and Pi bonds which is the stronger one Sigma bond is Stronger than pi bond

  • Question: In Water Molecule The Bond Angle Decreases From 109.28 To 104.5, Why This Does Happens?

    Answer :

    The repulsion between lone pair and lone pair electrons In NH3 molecule, the bond angle decreases from 109.28 to 107.3 why does it happen.The more repulsion between lone pair and bond pair.

  • Question: Ionic Compound Does Not Show The Property Of Space Isomerism, Give The Reason?

    Answer :

    Ionic bond is Electrostatic. It is non directional, so Ionic compounds does not show the property of Space Isomerism.

  • Question: What Are The Compounds Used For Extraction Of Gold And Silver?

    Answer :

    NaCN and KCN

  • Question: What Are The Favourable Conditions For Formation Of Cat Ions?

    Answer :

    1. Low Ionisational potential.
    2. Lesser Charge.
    3. More atomic size of atoms forms cations easily.
    4. Ions having Inert gases configuration formed easily.

  • Question: What Are The Favourable Conditions For Formation Of Anions?

    Answer :

    • High Electron affinity
    • Small size
    • Less charge of an atoms form anion more easily
  • Question: What Are The Important Sources Of Phosphorus?

    Answer :

    Sources of Phosphorous are Phosphate rocks

    • Flourapatite {2Ca3 (PO4)2 CaF2}
    • Phosphorite {Ca3 (PO4)2}
  • Question: What Are The Raw Materials Used For The Precipitation Of Na2co3 By Solvay Ammonium Process?

    Answer :

    Sodium Chloride, limestone and ammonia.

  • Question: What Are The Units Of Bond Length?

    Answer :

    The intermolecular distance between the bonded atoms in a molecule is called bond length and the units are Angstrom units (A0).

  • Question: What Can You Calculate By Selecting Born-¬haber Cycle?

    Answer :

    Born¬Haber cycle is useful for calculation of lattice energy, heat of reaction and electron affinity.

  • Question: What Does Ammonal Contain? For What Purpose It Is Used.

    Answer :

    It is a mixture of ammonium nitrate and aluminium powder is called ammonal, which is used as an explosive in Bombs.

  • Question: What Does One Debye Equals?

    Answer :

    10¬18 e.s.u – Cm

  • Question: What Happens If Lattice Energy Increases?

    Answer :

    • The Strength of ionic bond
    • Stability of the Ionic compound
    • Ease of formation of the Ionic bond Increases
  • Question: What Is Addition Reaction?

    Answer :

    When atoms (or) group of atoms are added, to form more saturated compound it is called addition reaction.

  • Question: What Is Banana Bond? Diborane Contains How Many Banana Bonds

    Answer :

    In Diborane B¬H¬B, bridge, which is formed by the sharing of two electrons, is called banana bond or Tau bond Diborane contains two banana bonds.

  • Question: What Is Co¬-ordination Number?

    Answer :

    The number of appositively charged ions surrounded a particular ion in an ionic crystal lattice is called co¬-ordination number.

  • Question: What Is Dipole?

    Answer :

    In polar covalent molecule, one atom gets positive charge and the other one gets negative charge hence called dipole.

  • Question: What Is Electrophilic Addition Reaction?

    Answer :

    When the attacking species during the addition reaction is Electrophile, it is called electrophilic addition reaction. 

    Ex; (+) (¬) (+) (¬) (+) (¬)

    CH2Br¬CH2BràH2C¬CH2+BrBr¬àH2C=CH2+Br2¬

  • Question: What Is Inert Pair Effect?

    Answer :

    The reluctances of ns electrons in the participation of bond formation is called inert pair effect.

  • Question: What Is Nucleophilic Addition Reaction?

    Answer :

    When the attacking species during the addition reaction is Nucleophilic, it is nucleophilic addition reaction.
     Ex; Acetaldehyde cynohydrin

  • Question: What Is Structure Of Nacl And Give The Co¬-ordination Number Of Nacl?

    Answer :

    The structure of Nacl is face centered Cubic and Co¬-ordination number of Nacl is 6.

  • Question: What Is Sublimation Energy?

    Answer :

    The amount of energy required to convert one mole of solid substance to vapor state is called Sublimation energy

  • Question: What Is The Formula Of Hypo In Oxidation State Of Sulphur?

    Answer :

    The formula of Sodium Thio Sulphate is Na2S2O3. H2O is called as hypo Oxidation state of Sulphur in it is +6 0r ¬2.

  • Question: What Is The Molecular Formula, Structure, And Bond Angle Of Phosphorous?

    Answer :

    • P4¬ Phosphorous molecule (Tetra atomic)
    • Structure ¬- Tetrahedral
    • Bond angle – (60 degrees)
  • Question: What Is The Order Of C¬h Bond Length In C2h6 And C2h4 And C2h2?

    Answer :

    c2h6>c2h4 >c2h2

  • Question: What Is The Relation Between Reaction Taking Place In One Of The Several Stages And The Total Amount Of Energy Liberated In The Reaction?

    Answer :

    The relation between reaction taking place in one of the several stages and the total amount of energy liberated in the reaction is same.

  • Question: What Is The Structure Of Cscl And Give The Co¬-ordination Number Of Cscl?

    Answer :

    The structure of cscl is Body Centered Cubic and the Co-ordination number of CsCl is 8.

  • Question: What Is The Super Phosphate Of Lime?

    Answer :

    Ca (H2Po4)2 caSO4. 2H2O. Super phosphate of lime is also called as Calcium super Phosphate Sixth group elements are called as.Chalcogens (ore forming elements)

  • Question: What Is Thermite Mixture?

    Answer :

    A mixture of Fe2O3 and ammonium powder in 3:1 ratio is called Thermite mixture.

  • Question: Which Are The Most Abundant Metal And Third Abundant Element In The Earth Crust?

    Answer :

    Aluminium (Al) (7.28%)

  • Question: Which Element In 1a Group Does Not Form Peroxides?

    Answer :

    Lithium

  • Question: Which Element In 1a Group Is Lighter Than Water?

    Answer :

    Lithium, Sodium, and potassium

  • Question: Which Elements Are Present In Electron?

    Answer :

    Electron consist of 95 % mg, 5% Zn.

  • Question: Which Group Of Elements Is Called Alkaline Earth Metals?

    Answer :

    1st A group because the oxides and hydrides are alkaline in nature Among 1A group elements why the element lithium is the most powerful, reducing agent in equivalent state Due to its low sublimation and hydration energy.

  • Question: Which Metal Is More Metallic In Nature Among 1a Group Elements?

    Answer :

    Francium.

  • Question: Which Property Among The Following Generally Decreases From Top To Bottom In A Group 1a?

    Answer :

    Electropositive, Density,Basic nature, classical reactivity Electro affinity, electro negativity, Ionisational potential,melting and boiling points decreases from top to bottom in a group 1A from lithium to caesium.

  • Question: Which Property Among The Following Generally Increases From Top To Bottom In A Group 1a?

    Answer :

    Electropositive, Density,Basic nature, classical reactivity Electropositive, Density, Basic nature, classical reactivity, and solubility generally increase from top to bottom from lithium to caesium.

  • Question: Which Theory Explains The Paramagnetic Nature Of Oxygen?who Proposed It?

    Answer :

    Molecular orbital theory, proposed by Hunds and Mulliken

  • Question: Which Univalent Element Cannot Form Alums And Why?

    Answer :

    Lithium (Li+) does not produce alums because of its very small size.

  • Question: Who Proposed Vsepr Theory? What Does It Explain?

    Answer :

    VSEPR theory was proposed by Sedgwick and Powell. It explains the shapes of polyatomic molecules.

  • Question: Why Nitrogen Cannot Form Penta Halides?

    Answer :

    Because of the absence of d¬orbital in its valency shells Ortho Phosphoric acid is. Give oxidation no of phosphorous in it.

    H3PO4¬ortho phosphoric acid Oxidation state of phosphorous is +5.